Differentiation of bacterial spores via 2D-IR spectroscopy

Ultrafast 2D-IR spectroscopy is a powerful tool for understanding the and dynamics of biological molecules in solution phase. A number recent studies have begun to explore utility information-rich spectra analytical applications. Here, we report application ultrafast detection classification bacterial spores. Bacillus atrophaeus thuringiensis spores as dry films on CaF2 windows were obtained. The sporulated nature bacteria was confirmed using diagonal off-diagonal peaks arising from calcium dipicolinate CaDP·3H2O biomarker sporulation. Distinctive peaks, protein amide I region spectrum used differentiate two types spore. identified marker modes demonstrate potential use methods direct means spore classification. We discuss these new results perspective with current state measurements, showing that exists apply detect surfaces suspensions well films. how applying screening methodologies would enable creation library purposes.